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KMID : 0043320160390040474
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Archives of Pharmacal Research
2016 Volume.39 No. 4 p.474 ~ p.480
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Identification of cytoprotective constituents of the flower buds of Tussilago farfara against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells
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Kang Un-Woo
Park Ji-Young
Han Ah-Reum
Woo Mi-Hee
Lee Je-Hyun
Lee Sang-Kook
Chang Tong-Shin
Woo Hyun-Ae
Seo Eun-Kyoung
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Abstract
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A new cytoprotective compound, 1-[(4S)-3,4-dihydro-4-hydroxy-2,2-dimethyl-2H-1-benzopyran-6-yl]-ethanone (1) was isolated from the flower buds of Tussilago farfara L. (Compositae), together with eight known compounds, 3,4-dicaffeoyl isoquinic acid (2), trans-cinnamic acid (3), 4-hydroxyacetophenone (4), 4,5-dicaffeoylquinic acid methyl ester (5), 3,5-dicaffeoylquinic acid methyl ester (6), 4-hydroxybenzoic acid (7), isoquercetrin (8), and ligucyperonol (9). Compounds 2?4 were found in this plant for the first time. The isolates 1?9, were tested for their cytoprotective activities against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells. Among them, 1 and 3 showed significant cytoprotective activities as determined by MTT assay and lactate dehydrogenase leakage, indicating their possibility as the potent cytoprotective agents. The structure of 1 was determined by spectroscopic data analysis including 1D- and 2D-NMR experiments, and its absolute configuration was elucidated by a circular dichroism.
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KEYWORD
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Tussilago farfara, Compositae, Phenolic compound, Cytoprotection, Oxidative stress
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